# SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. A standard 96 well plate was set up as described in Protocol Table 4. 2. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. 17,000 cells. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. 25% and 0. These robust readers have been placed in labs from Antarctica to. The equation that allows one to calculate absorbance from % transmittance is. % transmittance of plates used in a plate reader. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Optimized for fluorescence microplate readers. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Well scanning With well scanning, multiple points within each well can be read, providing a high. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. 3 OD at 200 nm. x. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Source plate capacity; 1 x plates. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The luminescence was measured in a SpectraMax. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Bay Gray powder coated metal back plate, column, and base. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. #C1500) Control standard endotoxin (ACC cat. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Controlled by the computer. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. 2. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Dark blue indicates well will be read. Category 2 and 3 microplate readers use gradient heating as well. From the dropdown Menu, select Wellplates. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Or scan up to 384 wells at any time to determine the best assay conditions. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Ensure that cells reach around 80–90% of confluence on the day of the. TNF standard curve. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. 190. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. It also integrates with the powerful SoftMax Pro Software for. 9000-0756. Selects either 96-well or 384-well plate for a stand-alone read. These plates are compatible with standard fluorescence microplate readers and are offered in a. We would like to show you a description here but the site won’t allow us. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). 45 µm PVDF. Specifications subject to change without notice. Microplate readers, SpectraMax®, with SpectraDrop. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Inconsistent results: cannot determine whole well confluence – well after well. Learn more. Compare specifications and request a quote from leading suppliers. x. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 1. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Published Dec 16, 2020 | Updated Jun 22, 2023. the Molecular Devices SPECTRAmax® 190 and 250. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Part of a plate can be selected so that only a selected area of the plate. Plus 384. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. . 5 OD at 492 nm Computer interface USB 2. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Corning. Growth medium was then replaced with fatty acid uptake solution. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Microplate Readers include the M3, M4, M5, and M5e models. These robust readers have been placed in labs from Antarctica to the. Cayman Chemical. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 312 gain calibration failed. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 3. x. Published Dec 16, 2020 | Updated Nov 07, 2022. 1 fmol luciferase per well. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Unparalleled performance on a personalized platform. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. 50/plate. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 1 x plates; Instrument dimensions. In SoftMax Pro, Dual Read mode was selected. x. x. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. 2% in. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Published Dec 16, 2020 | Updated Dec 06, 2021. Order Now. 5032338. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 40266. 1 – 2. the Gemini XPS reader. Two different agonists, acetylcholine and carbachol, were tested. Integrated stacker option. Microplate Readers include the M3, M4, M5, and M5e models. Multimode microplate reader equipped with latest detections like fluorescence,. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. SpectraMax i3x Multi-Mode Microplate Reader. A. Our. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Wavelength(s):. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. Page 1 of 8 . Compare this item. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. T = I/I 0 and %T = 100 (T). 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 9. Or scan up to 384 wells at any time to determine the best assay conditions. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. 1. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 2 OD at 200 nm; this decreased to 0. The StakMax stacker also allows users to feed plates into the loading area manually. PURPOSE. 2. Running both standard spectrophotometer and microplate. Four wash/rinse bottles are included and configurable for both 96- and 384. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. M5. S. measurements in compatible Molecular Devices® microplate readers. Top and bottom reads are. 315 can't find zero order. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 0200-6262. #3693; n = 4 for each standard). Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Click Add to create the plate. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Sample by eye within . Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 7°C and the bottom to 36. Temperature control: 4°C above ambient to 45°C. 4 (Molecular Devices cat. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. #C1500) Control standard endotoxin (ACC cat. 96-, 384-, and 1536-well microplates. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Selects either 96-well or 384-well plate for a stand-alone read. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Cell viability and cytoxicity assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Wells to read. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Values are averages from 5 clean plates, each filled with 200 µL water/well. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. UV absorbance detection. The. Published Dec 16, 2020 | Updated Dec 16, 2020. #23225). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. #ABS Plus) Cell plating and treatment prior to assay. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 00 Add to cart; Molecular Devices EMax Microplate Reader $. Lucid Scientific. Step 3: Click “Settings” and a screen will appear (Figure 1). Description. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Making it possible to perform kinetic analysis of solid. Molecular Devices L-Max II Luminescence Microplate Reader. A control well was set up to verify. Automated live cell imaging – cell counting and confluence. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. Fluorescence Microplate Reader Comparison Chart. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. No accessories are needed and SoftMax® Pro Software. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 000 OD ± 1. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Contents of wells were pipetted up and down several times to mix. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 5°C at 37°C. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. External Dimensions without lid (mm). Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. #CA961) Methods. M2. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Microplate Reader. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. What are the dimensions of the plate? Code. 384-WELL SBS STERILE MICROPLATE. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The variations include adjustable. 000 OD ± 1. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Use standard plate unless you see your type listed. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. We would like to show you a description here but the site won’t allow us. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 0% ± 0. Materials. 3% in white 96- and 384. 00 USD. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Products : General, FilterMax F5 reader. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Click SoftMax Pro Software User Guide. • Easy and gentle immobilization of biotin. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. $488. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. This can increase throughput and efficiency, thus saving time and space in the lab. Make sure toPlate reader instructions Computer. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. a white 96-well half-area microplate (Corning cat. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. 100 fmol luciferase per well. When the user applies a set temperature of 37°C, the top is heated to 37. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The plate reader can measure multiple readouts, including UV and. Mix samples in microplate wells by user-programmable Automix feature. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. CO 2 & O 2 control. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. They are also sealable using different types of adhesive mats foils or films. 130009WMicroplate Readers. Detection of KASP genotyping reactions. Selects either 96-well or 384-well plate for a stand-alone read. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. For biochemical assays, the washer removes unbound material and unreacted reagents. Microplate Readers. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 500 USD. EMax. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. x. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. 96-well microplate compatible Well read area range: D4. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. robotics-automation-compatible. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The advanced design of the Sunrise plate reader includes. style. Clear plates will give more cross talk between wells. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. x. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 1 mL/well of media. Two high-energy xenon flash lamps: Reading capabilities. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Published Dec 16,. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). absorbance microplate reader GEA. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. 0% ± 0. Intuitive graphical user interface makes each assay extremely easy to set. The system features 96, 384,. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. 20 µL (96-well plates) or 10 µL (384. #SpectraMax L Config) Methods. Bioz Stars score: 86/100, based on 40 PubMed citations. The color touch screen provides a visual user interface, making programming fast and intuitive. 25, 3. Compare. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. , Corning, NY) or Greiner. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. 5 second read per plate. 005 OD Normal and Rapid modes, 96- and 384-well plates;. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. x. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. 2b,c. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 000 to 2. Compare this item. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. All trademarks used herein are the property of. Dual injectors for kinetic assays. 96-, 384-, and 1536-well microplates. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Gemini XPS Reader scans. Published Dec 16, 2020 | Updated Dec 16,. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. p-Nitroaniline - Catalog No. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,.